Shi Ying Tor

Institute of Molecular and Cell Biology (IMCB), Brain Plasticity Group (BPG), Fu Yu Lab

• Routine maintainence of mouse colony lines through tagging and tailing, genotyping (PCR, gel electrophoresis) and weaning of mice in breeding cages to ensure sufficient stock
• Transcardiac perfusion to obtain mouse brain tissue, cryosectioning of fresh-frozen and fixed-frozen tissue, mounting of free-floating sections
• Immunohisotchemistry (IHC) staining of free floating sections
• Fluorescent In Situ Hybridisation (FISH) Kit V1 and V2 (Fresh Frozen and Fixed Frozen)
• Cubic Clearing for Light Sheet Imaging
• Behavioural Experiment (Contexual Feeding Conditioning (CFC), Tube Dominance Test, Binge-induced Compulsive eating, Fast Refeeding)
• Imaging Tools and Techniques ( Confocal, Zyla sCMOS, EVOS Microscope, Image Processing Tools:ImageJ/ Fiji)
• Stereotaxic Injection of Viral Vectors and Stem Cells
• Gut, Brain, Organ Harvesting of Mouse for Proteomics studies (Gut Cell isolation for single cell sequencing)
• Proteomics study of different brain regions
• Cell culturing, cell seeding, bacteria transformation, ligation to plasmid, restriction digest, DNA extraction from gel, Miniprep and Maxiprep
• Blood Collection (Retro-Orbital, Submandibular)
• Assist in specialised procedure (Celiac ganglion and nodule ganglion harvesting, Bariatric surgery, Vagotomy, Pyloroplasty)
• Click chemistry for Proteomics study
• Procurement of Lab Materials
• Training of New Staff and Interns
• Planned and initiated research study protocols, implemented and administered data collection and analysis systems.

• Based on order form sent in by doctors, organise and schedule dialysis session based on the patient's schedule and availability of beds in the centre
• Experience using medical records and databases and have knowledge of relevant medical terminology
• Effective communication between nurses and doctors to adapt to any changes in patient's schedule or prioritise patient with urgent condition and send staff out for suspected cases of COVID-19

• Taught Primary 4 and 5 students English, Math and Science via hands on activities such as conducting experiments to allow them to grasp concepts easily
• Innovate different approaches to solve a question to allow students to understand a problem better
• Interpersonal skills through communication with students to develop relationship that provides them with a condusive learning environment

Bachelor's Degree in Biomedical Science with Honours

(Obtained First Class Honours)

Holding school at Management Development Institute Singapore (MDIS)

• Relevant modules: Cellular and Immunological Methods, Medical Microbiology, Metabolic Biochemistry, Cell Biology and Genetics
• Final Year Project on Traditional Chinese Medication effects on Commensal Gut Microbiome

Awarded International Leadership Scholarship
Dissertation Project on Spinal Cord Dorsal Horn Projection Neurons under Professor Andrew Todd's lab