Junyi Liu
Summary
Graduating PhD student with abundant experiences of stem cell culture (including high throughput culture), guided differentiation, and relevant molecular biology characterization techniques. Successfully contributed to multiple research projects, which demonstrated problem-solving, learning, and professional skills that can ensure high-quality and reliable research outcomes.
Overview
6
6
years of professional experience
Work History
Student Work Scheme Research Assistant
Nanyang Technological University
01.2025 - 07.2025
- Participate in the project of "Combinatorial organoid screening for establishing advanced iPSC derived lung organoids" novel protocols to improve human lung organoid differentiation and maturation.
- Conduct stem cell and organoid culture; conduct molecular biology analyses (qPCR, FACS, IF staining etc.); analyze and present experimental data; discuss and design experiments with senior researcher; perform research-related administrative tasks and assist in day-to-day management of laboratory.
- Mentored junior research interns in laboratory practices and data analysis, interpretations, and presentation.
PhD Programme
Nanyang Technological University
01.2021 - 01.2025
- Fully defined GelMA hydrogel for mouse small intestinal organoid culture allows high-throughput screening applications.
- Developed novel and fully defined GelMA-based hydrogel that could support organoid growth comparable to that cultured in “gold-standard” Matrigel.
- Developed a high-throughput organoid culture platform with totally defined hydrogel for highly efficient screening applications.
MRes (Master of Research) Program
Imperial College London
09.2019 - 08.2020
- RBioinformatics prediction (R language) and in vitro validation of potential circRNA-miRNA-mRNA regulatory network under hypoxia conditions.
Summer Intern
University of Bristol Research Labs
06.2019 - 07.2019
- Shadowed and performed RNA related lab work (e.g. RNA purification, qPCR etc.) to help identify homozygous/heterozygous mutated animals.
- Helped with optimizing calcium staining protocols in zebrafish larvae, learnt how to breed zebrafish in lab environment and perform in-vivo experiments (zebrafish specifically).
Education
Ph.D. - Regenerative Medicine
Lee Kong Chian School of Medicine, NTU
Singapore01-2026
MRes - Biomedical Research
Imperial College London
London, United Kingdom08-2020
BSc - Physiological Science
University of Bristol
Bristol, United Kingdom06-2019
Skills
Biological Science Relevant
- Organoid culture (mouse small intestine, human lung, human skin/sebaceous gland)
- Cell culture (multiple iPSC lines, HEK293, HUVEC)
- RT-qPCR
- ELISA
- Immunofluorescent staining
- Confocal microscopy
- Flow cytometry
- Celleste 6 Image analysis software
Material Science Relevant
- Hydrogel fabrication (photo-, chemical-, ionic-crosslinking, eg GelMA, PEG-4MAL, alginate)
- Rheology
- Swelling ratio (experienced with lyophilization)
- Degradation study
- SEM imaging
- Basic 3D CAD design of microfluidics chips
- Microfluidics and high-throughput cell encapsulation with hydrogel
- COPAS large particle sorting
Patents and Publications
Choo Y., Irvine A. S., Neethu Govindan Kutty Devi, Htwe S. S., Liu J. SYSTEM AND METHOD FOR FORMING MICROPARTICLES FOR CULTURING ORGANOIDS. The patent application has progressed to PCT procedure (PCT/SG2024/050598)
Additional Information
Poster presentation at 12th World Biomaterials Congress (WBC2024) with the title of: Liu J., Neethu Govindan Kutty Devi, Irvine A. S., Htwe S. S., Tan L. P., Choo Y. A platform technology for high-throughput organoid culture in totally defined ECM microcapsules.
Languages
English
Bilingual or Proficient (C2)
Chinese (Mandarin)
Bilingual or Proficient (C2)
Timeline
Student Work Scheme Research Assistant
Nanyang Technological University
01.2025 - 07.2025
PhD Programme
Nanyang Technological University
01.2021 - 01.2025
MRes (Master of Research) Program
Imperial College London
09.2019 - 08.2020
Summer Intern
University of Bristol Research Labs
06.2019 - 07.2019
Ph.D. - Regenerative Medicine
Lee Kong Chian School of Medicine, NTU
MRes - Biomedical Research
Imperial College London
BSc - Physiological Science
University of Bristol
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