Summary
Overview
Work History
Education
Skills
Additional Information
Websites
Awards
Affiliations
Publications
Timeline
Generic
Su Su Htwe

Su Su Htwe

Summary

Experienced scientist with 11+ years in molecular cell biology, immunology, and organoid research. Proficient in research methods, data analysis, mentoring, and project management. Skilled in lab techniques and scientific writing, committed to advancing knowledge and making impactful contributions to the scientific community.

Overview

10
10
years of professional experience

Work History

Senior Post Doctoral Research Fellow (Full Time)

Lee Kong Chain School of Medicine, Stem cell and Regenerative Medicine, NTU Singapore
09.2022 - Current

Project: Guided niche reconstruction for advancing organoid system using large-scale combinatorial screening system (iPSC-derived lung Organoids)

  • Developed and optimised a high-throughput organoid screening system for niche discovery using Combicult Technology
  • Development of a large-scale microencapsulation system (patented)
  • Establishment of a high-throughput organoids sorting system using a large particle sorter
  • Maintenance and expansion of different iPSC lines
  • Technical Transfer and patent filing
  • Mentored junior researchers, fostering an environment of professional growth and academic excellence
  • Developed grant proposals, securing necessary funding for research projects
  • Collaborated with interdisciplinary teams for project milestones
  • Lab management
  • Authored professional scientific papers for publishing in peer-reviewed journals

Senior Post Doctoral Research Fellow

Bioinformatics Institute (BII), A*STAR, Singapore
04.2022 - 09.2022

Development of Machine Learning Based phenotypic based assay

  • Optimised the ex-vivo PDx cell model for screening.
  • Automatic handling of cell culture for high throughput applications
  • Developing high throughput imaging based phenotypic profiling method to assess drug response.
  • Project management


Post-Doctoral Research Fellow (full time)

Bioinformatic Institutes (BII), A*STAR, Singapore
03.2018 - 04.2022

Development of Transcriptomic based new approach method (NAM) for safety assessment

  • Optimise cell model for potency assessment including NOAEL and LOAEL
  • Transcriptomic Assay using TempO-SEQ technique
  • Led complex cellular phenotypic analysis projects, ensuring high-quality data collection and interpretation.
  • Conducted thorough literature reviews, synthesizing relevant information to inform research hypotheses and methodologies.
  • Collaborated with interdisciplinary teams for holistic research approaches and comprehensive results.
  • Mentored junior researchers, fostering an environment of professional growth and academic excellence.
  • Managed complex datasets, ensuring accuracy and consistency in data collection and analysis processes.
  • Responsible for data and methodology sharing and progress update

Researcher (part- Time)

Division of Immunology, University of Nottingham
01.2015 - 01.2017

Project: Innovative Immunoregulatory Implant - IMMODGEL (EU-FP7 and Horizon2020 collaborative) project

  • Developed a platform for the study of immunomodulation against different implanted biomaterials with different topographies
  • Peripheral blood mononuclear cells isolation, different coculture model, immunostaining
  • Established Foreign body on chip (organ on chip) model and lung on chip model
  • Presented findings at international conferences, effectively communicating complex research outcomes to diverse audiences.
  • Developed protocols for sample collection and processing, streamlining workflows, and increasing efficiency within the lab environment.
  • Collaborated with multidisciplinary teams to analyze complex datasets, contributing to significant advancements in immunology research.
  • Report deliverables, Scientific reporting
  • Responsible for data and methodology sharing and progress update
  • Procurement
  • GLP practice and training
  • Risk assessment

Education

PhD - Biomedical Sciences

University of Nottingham
UK
01.2017

MSc - Immunology and Allergy

University of Nottingham
UK
01.2012

Bachelor of Medicine, Bachelor of Surgery -

University of Medicine 1
Myanmar

Skills

Cell culture

  • Primary (patient-derived) and different Cell lines (> 20 adherent and suspension cell lines- lung, liver, kidney, skin, immune cells)
  • HiPSC and HESC ( Stem cell expansion and differentiation)
  • Immune Cell Culture including PBMC isolation
  • Lung Organoids (iPSC-based lung organoid differentiation) and GI organoids (Lgr5 progenitor differentiation )
  • Complex Cell Culture System
  • Organ on chip: Lung on chip, foreign body on chip
  • 3D hydrogel cell encapsulation (Gelatin, Matrigel, Gel-MA, collagen) using chemical and photo crosslinking
  • Air-liquid interface cell model
  • Automated High- throughput cell culture for drug and small molecules screening
  • High-throughput organoid generation for screening using COPAS large particle sorting

Microscopy

  • Confocal microscopy, Fluorescence microscopy, SEM microscopy
  • Multiplex Immunostaining
  • Imaging software- Image J, Zeiss, Imaris, Cell Xpress for high throughput screening

Molecular Biology Techniques

  • Transfection, Gene Knock down (SiRNA, CRISPR)
  • scRNA-seq analysis
  • Real time Polymerase Chain Reaction
  • Transcriptomic assay (TempO-SEQ)
  • Western blotting
  • Flow cytometry
  • Enzyme Linked Immunosorbent Assay
  • Cell Based assay (Luminescence and Fluorescence)
  • Hydrogel Encapsulation of cells

Statistics and Bioinformatics

  • Bioinformatics - Pathway enrichment tool: David functional Annotation tools, Reatome pathway analysis
  • R programming
  • GraphPad Prism (Statistics)
  • Microsoft office, Inkscape, Gimp

Supplementary training

  • Ethics of human tissue and animal studies in research
  • Project Management Course
  • IP Fundamentals in business
  • Demystifying the patent application process

Additional Information


A platform technology for high-throughput organoid culture in totally defined ECM microcapsules

Junyi Liu, Su Su Htwe, Neethu Govindan Kutty Devi, Scott A. Irvine, Lay Poh Tan, Yen Choo

World Biomaterials Congress (2024), Daegu, Korea

A Platform Technology for combinatorial organoid screening for the Discovery of Tissue-Specific Niche Signals in regenerative medicine Su Su Htwe, Yen Choo.

Singapore Cell and Gene Therapy Conference, Singapore, 2025A three dimensional culture system for investigating inflammatory responses in human lung S S Htwe, H Harrington, A J Knox, F R A J Rose, J W Aylott, J W Haycock and A M Ghaemmaghami 9th World Congress on Alternatives and Animal Use in the Life Sciences (August 2014), Prague, Czech Republic

Toward Mechanistic-Based Assessment: Rapid Elucidation of the Key Molecular Pathways underlying Diketone-Induced Bronchiolitis Obliterans Using Phenotypic and Transcriptomic Profiling S. S. Htwe, K. W. Kong, S. O. Fu, J. C. Kong, V. Limviphuvadh, B. P. Smith, S. Maurer-Stroh, S. Hoon, and L. Loo. 59th Annual Meeting of the Society of Toxicology (SOT) (2020), Anaheim, California

Role of ROCK Isoforms in Regulation of Stiffness Induced Myofibroblast Differentiation in Lung Fibrosis Su Su Htwe, A J Knox, A M Ghaemmaghami American society for cell biology meeting (December 2016), San Francisco, USA

Paper-based in-vitro model for on-chip investigation of the human respiratory system Rahim Rahimi, Su Su Htwe, Manuel Ochoa, Ali Tamayol, Ali Khademhosseini, Amir Ghaemmaghami, and Babak Ziaie Hilton Head Workshop 2016: A Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2016

Foreign body on a chip to study implant host immune responses Yu Shrike Zhang, Su Su Htwe, NE Vrana, Amir Ghaemmaghami, M Dokmeci, Ali -Khademhosseini TERMIS EU Congress (2017), Darvos, Switzerland

A Micro-engineered Biomimetic Hydrogel for Studying the Role of Extracellular Matrix Stiffness in Lung Fibrosis Su S Htwe, João Ribas, Alan J. Knox, Ali Khademhosseini, Amir Ghaemmaghami TERMIS World Congress (2015), Boston, MA, USA

Micro engineered hydrogel for 3D culture of human lung fibroblasts S S Htwe, A J Knox, A M Ghaemmaghami M5 biomedical Imaging Conference, School of Veterinary Medicine and Science, University of Nottingham, UK (2014)

In vitro lung Fibrosis model S S Htwe, A J Knox, A M Ghaemmaghami Post Graduate Research Symposium, School of Life Science, and University of Nottingham, UK (2015)

Programming of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation J Kzhyshkowska, V Riabov, F Salazar, SS Htwe, A Gudima, C Schmuttermaier, J Barthes, H Knopf-Marques, H Klüter, A Ghaemmaghami, E Vrana TERMIS EU Congress (2017), Darvos, Switzerland


Oral Presentations

A 3D culture system for investigating inflammatory responses in human lung Young Scientists Award Oral Presentation, 9th World Congress on Alternatives and Animal Use in the Life Sciences (August 2014), Prague, Czech Republic

Differential NF-KB activation in lung fibroblasts in 2D and 3D culture system

Immunology Showcase, February 2016, University of Nottingham, UK


Awards

  • 2016 Early Researcher of Excellence Award- in recognition of outstanding research contributions from University of Nottingham, 2016
  • Biotech Travel Award to attend ASCB annual meeting, San Francisco, USA, 2016, 2015
  • Post Graduate Travel Award, University of Nottingham (2016), 2015
  • Young Scientist Travel Award from 2015 4th TERMIS World Congress, Boston, MA, USA, 2014
  • Vice Chancellor Research Excellence (International) PhD Scholarship from University of Nottingham, UK, 2014
  • Young Scientist Travel Award from 9th World Congress on Alternatives and Animal Use in the Life Sciences (August 2014), Prague, Czech Republic, 2012
  • Distinction in MSc immunology and Allergy, University of Nottingham, UK

Affiliations

  • Member of Singapore Stem Cell Symposium (2022- present)
  • Member of British Society for Immunology (2011-2019)
  • Member of IEEE Engineering in Medicine and Biology Society (2015-2017)
  • American Society for Cell Biology (2016-2017)
  • Member of TERMIS (2011-2017)
  • Member of Society of Toxicology (SOT) (2019-2020)

Publications

  • Htwe SS, Cha BH, Yue K, Khademhosseini A, Knox AJ, Ghaemmaghami AM. Role of rho-associated coiled-coil forming kinase isoforms in regulation of stiffness-induced myofibroblast differentiation in lung fibrosis. American Journal of Respiratory Cell and Molecular Biology 2017;56(6):772-783.
  • Htwe SS, Harrington H, Knox A, Rose F, Aylott J, Haycock JW, Ghaemmaghami AM: Investigating NF-kappaB signaling in human lung fibroblasts in 2D and 3D culture systems. Respiratory research 2015, 16:144.
  • Rahimi R, Htwe SS, Ochoa M, Donaldson A, Zieger M, Sood R, Tamayol A, Khademhosseini A, Ghaemmaghami AM, Ziaie B: A paper-based in vitro model for on-chip investigation of the human respiratory system. Lab on a Chip 2016, 16:4319-25.
  • Sharifi, F, Htwe, S. S., Righi, M., Liu, H., Pietralunga, A., Yesil-Celiktas, O., Maharjan, S., Cha, B.-H.; Shin, S. R., Dokmeci, M. R, Vrana, N. E., Ghaemmaghami, A. M., Khademhosseini, A., Zhang, Y. S. A Foreign Body Response-on-a-Chip Platform. Adv. Healthc. Mater. 2019, 8 (4), 1801425.
  • Riabov V, Salazar F, Htwe SS, Gudima A, Schmuttermaier C, Barthes J, Knopf-Marques H, Klüter H, Ghaemmaghami AM, Vrana NE, Kzhyshkowska J: Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation. Acta biomaterialia 2017, 53:389-98.
  • Knopf-Marques H, Singh S, Htwe SS, Wolfova L, Buffa R, Bacharouche J, Francius G, Voegel J, Schaaf P, Ghaemmaghami AM, Vrana NE, Lavalle P: Immunomodulation with Self-Crosslinked Polyelectrolyte Multilayer-Based Coatings. Biomacromolecules 2016, 17:2189-98.
  • Singh S, Awuah D, Rostam H, Emes R, Kandola NK, Onion D, Htwe SS, Rajchagool B, Cha B-H, Kim D, Tighe P, Vrana NE, Khademhosseini A, Ghaemmaghami AM: Unbiased Analysis of the Impact of Micropatterned Biomaterials on Macrophage Behaviour Provides Insights beyond Pre-defined Polarisation States. ACS Biomaterials Science & Engineering 2017.

Timeline

Senior Post Doctoral Research Fellow (Full Time)

Lee Kong Chain School of Medicine, Stem cell and Regenerative Medicine, NTU Singapore
09.2022 - Current

Senior Post Doctoral Research Fellow

Bioinformatics Institute (BII), A*STAR, Singapore
04.2022 - 09.2022

Post-Doctoral Research Fellow (full time)

Bioinformatic Institutes (BII), A*STAR, Singapore
03.2018 - 04.2022

Researcher (part- Time)

Division of Immunology, University of Nottingham
01.2015 - 01.2017

PhD - Biomedical Sciences

University of Nottingham

MSc - Immunology and Allergy

University of Nottingham

Bachelor of Medicine, Bachelor of Surgery -

University of Medicine 1
Su Su Htwe