Enthusiastic, eager to contribute to team success through hard work, attention to detail and excellent organizational skills. Clear understanding of tasks . Motivated to learn and grow. Results-driven professional seeking position as Research Assistant. Focused on performance and flexible with specific needs of different assignments.
- Cell counting, Injection of pathogen in fishes.
Media Preparation (hydroponics, microbial, plant)
Gel Electrophoresis (SDS PAGE, Native PAGE, AGE), Chromatography (Column, TLC, HPLC)
UV-Spectrophotometric and Colorimetric Techniques
DNA Purification Techniques
Extraction, Quantitative and Qualitative analysis of DNA, RNA/plasmids from plant, animal and microbial cells
PCR (colony, sequencing reaction, cDNA reaction), ELISA
Gel extraction
PCR Purification
Plant Tissue Culture, Animal Tissue Culture
Genotyping and crossing of Arabidopsis mutant
Recombinant DNA Technology
Immunological Techniques (antigen antibody interaction, blood group determination, Hemocytometer)
Microbial Identification
Biophotometer
Chlorophyll extraction and estimation
TFC (Total Flavonoids Content), TPC (Total Phenolic Content) Antioxidant assay, NMN assay, Nitrate assay
Quantification of protein and phosphate assay
Molecular cloning (Ligation, transformation, screening)
Sequencing analysis
Agrobacterium Transformation
Competent Cell Preparation
IPTG induction
Gateway LR cloning Reaction
Western Blotting
Restriction digestion
Crispr /Cas9 (sgRNA Design, Vector Construction)
Agrobacterium transformation into plants( floral dipping)
GUS staining
Lyophilization (bench top freeze dryer)
Dr.Wayne Lee Forday
Senior Advisor / The School of Life science and Chemical Technology
Ngee Ann Polytechnic Sg 599489
Phone No. 91147100
Email. wayneforday@gmail.com
Publication
1. Effects of Storage Temperatures on Nitrogen Assimilation and Remobilization during Post-Harvest Senescence of Pak Choi.
Biomolecules 13, 10: 1540. https://doi.org/10.3390/biom13101540
2.Early detection of N, P, K deficiency in Choy Sum using hyperspectral imaging-based spatial spectral feature mining.
Frontiers in Photonics. Volume 5 - 2024,
https://doi.org/10.3389/fphot.2024.1418246
3.Advanced growth device boosts hydroponic efficiency: Enhancing yield and quality while reducing crop production cost and algae contamination. - Under revision
Environmental Technology & Innovation.
Experimental cooking
Reading
Dr.Wayne Lee Forday
Senior Advisor / The School of Life science and Chemical Technology
Ngee Ann Polytechnic Sg 599489
Phone No. 91147100
Email. wayneforday@gmail.com