- Performed LC-MS and LC-MS/MS for peptide mapping, PTM identification, molecular weight confirmation, and structural integrity assessment.
- Utilized TOF and Q-TOF platforms with UNIFI and MassHunter Qualitative Analysis software to identify glycosylation, oxidation, and other modification sites in antibodies and proteins.
- Conducted structural analysis, sequence alignment, and modification identification using Expasy, Uniprot, PDB, and CLUSTALW.
- Established antibody fragmentation workflows using IdeS and IdeZ enzymatic digestion (Fc/2 + F(ab')₂).
- Performed protein structural and glycosylation analysis using Glyserias enzyme digestion, assessing conformational stability.
- Evaluated protein main peaks and isoforms using Intact Mass and SEC-HPLC to monitor aggregate content and stability.
- Provided analytical support for multiple antibody/recombinant protein drug development projects and progress reports.
- Skilled in HPLC, UPLC, ELISA, SDS-PAGE, CE-SDS, and Western blot.
- Assisted in method validation, SOP revisions, and lab compliance with data traceability standards.
Protein Analysis Researcher
WuXi Biologics
08.2022 - 09.2023
- Expert in LC-MS/MS for primary structure confirmation, PTM analysis, and glycan profiling.
- Performed reduction and enzymatic digestion of monoclonal antibodies, achieving >80% sequence coverage.
- Analyzed glycan distribution using PNGase F digestion coupled with LC-MS.
- Operated Titer (affinity chromatography), HPLC, and performed routine column maintenance.
- Independently managed client projects, including sample testing, data analysis, and report generation.
International School Science Teacher (Primary) at Siam Singapore International SchoolInternational School Science Teacher (Primary) at Siam Singapore International School