SANTOSH KUMAR SRIVASTAVA

My research centers on Synthetic Biology, a cutting-edge discipline where I meticulously engineer living organisms for revolutionary advancements in human health and industrial applications. Employing advanced techniques, I manipulate genetic components to design and optimize biological systems with precision. My primary interests lie in creating innovative solutions to human health challenges through the engineering of probiotics and other beneficial microbes, as well as developing industrially relevant organisms for the efficient production of valuable biomolecules.

• Mentored two master and one undergrad students to complete the dissertation at Indian Institute of Technology India
• Mentored one master students to complete the dissertation at Memorial University Newfoundland St. John's Canada
• As a Postdoc, I am working with the research team at SynCTI NUS, Singapore, assisting the research associates in executing experiments to engineer probiotics aimed at improving endurance performance and reducing fatigue in warfighters. This project is funded by the Ministry of Defense in Singapore and the Air Force Research Laboratory (AFRL) in Ohio, USA

1. Srivastava, S.K., Foo, G.W., Aggarwal, N., Chang, M.W., 2024. Organ-on-Chip Technology: Opportunities and Challenges in Biomedical Research. Biotechnology Notes( Review Accepted )

2. Dhyani, R., Srivastava, S.K., Shankar, K., Ghosh, T., Beniwal, A. and Navani, N.K., 2021. A chemical genetic approach using genetically encoded reporters to detect and assess the toxicity of plant secondary metabolites against bacterial pathogens. Journal of Hazardous Materials, 418, p.126399. [IF: 14.2]

3. Shaikh, A.A., Nothias, L.F., Srivastava, S.K., Dorrestein, P.C. and Tahlan, K., 2021. Specialized metabolites from ribosome engineered strains of Streptomyces clavuligerus. Metabolites, 11(4), p.239. [IF: 5.5]

4. Bhando, T., Bhattacharyya, T., Gaurav, A., Akhter, J., Saini, M., Gupta, V.K., Srivastava, S.K., Sen, H., Navani, N.K., Gupta, V. and Biswas, D., 2020. Antibacterial properties and in vivo efficacy of a novel nitrofuran, IITR06144, against MDR pathogens. Journal of Antimicrobial Chemotherapy, 75(2), pp.418-428. [IF: 5.7]

5. AbuSara, N.F., Piercey, B.M., Moore, M.A., Shaikh, A.A., Nothias, L.F., Srivastava, S.K., Cruz-Morales, P., Dorrestein, P.C., Barona-Gómez, F. and Tahlan, K., 2019. Comparative genomics and metabolomics analyses of clavulanic acid-producing Streptomyces species provides insight into specialized metabolism. Frontiers in Microbiology, 10, p.2550 [IF: 5.2]

6. Srivastava, S.K., King, K.S., AbuSara, N.F., Malayny, C.J., Piercey, B.M., Wilson, J.A. and Tahlan, K., 2019. In-vivo functional analysis of a class A β-lactamase-related protein essential for clavulanic acid biosynthesis in Streptomyces clavuligerus. PloS One, 14(4), p.e0215960 [IF: 3.7]

7. Ghosh, T., Srivastava, S.K., Gaurav, A., Kumar, A., Kumar, P., Yadav, A.S., Pathania, R. and Navani, N.K., 2019. A combination of linalool, vitamin C, and copper synergistically triggers reactive oxygen species and DNA damage and inhibits Salmonella enterica subsp. enterica Serovar Typhi and Vibrio fluvialis. Applied and Environmental Microbiology, 85(4) [Equal contribution] [IF: 5.0]

8. Bown, L., Srivastava, S.K., Piercey, B.M., McIsaac, C.K. and Tahlan, K., 2018. Mycobacterial membrane proteins QcrB and AtpE: roles in energetics, antibiotic targets, and associated mechanisms of resistance. The Journal of Membrane Biology, 251(1), pp.105-117 [Equal contribution] [IF: 2.4]

9. Gupta, S.K., Srivastava, S.K., Sharma, A., Nalage, V.H., Salvi, D., Kushwaha, H., Chitnis, N.B. and Shukla, P., 2017. Metabolic engineering of CHO cells for the development of a robust protein production platform. PloS One, 12(8), p.e0181455.. [Equal contribution] [IF: 3.7]

10. Srivastava, S.K., Iyer, V.R., Ghosh, T., Lambadi, P.R., Pathania, R. and Navani, N.K., 2016. Isolation of a non-genomic origin fluoroquinolone responsive regulatory element using a combinatorial bioengineering approach. Nucleic Acids Research,44(5), pp.2451-2461. [IF: 19.2]

11. Srivastava, S.K., Lambadi, P.R., Ghosh, T., Pathania, R. and Navani, N.K., 2014. Genetic regulation of spy gene expression in Escherichia coli in the presence of protein unfolding agent ethanol. Gene, 548(1), pp.142-148. [IF: 3.9]

12. Patil, S.D., Sharma, R., Srivastava, S.K., Navani, N.K. and Pathania, R., 2013. Downregulation of yidC in Escherichia coli by antisense RNA expression results in sensitization to antibacterial essential oils eugenol and carvacrol. PloS One, 8(3), p.e57370. [IF: 3.7]

13. Ghosh, I.N., Patil, S.D., Sharma, T.K., Srivastava, S.K., Pathania, R. and Navani, N.K., 2013. Synergistic action of cinnamaldehyde with silver nanoparticles against spore-forming bacteria: a case for judicious use of silver nanoparticles for antibacterial applications. International Journal of Nanomedicine, 8, p.4721. [IF: 7.0]

Aggarwal, N., Koh, E.W., Srivastava, S.K., Sieow, B.F. and Hwang, I.Y., 2022. Microbiome Engineering for Metabolic Disorders. Principles in Microbiome Engineering, pp.47-91.

• Engineering of a probiotic to modulate the gut-brain axis 2023. Srivastava S.K., Foo G.W., Hwang I.Y., Wun K.S., Chang M.W., Conference International Metabolic Engineering Society - AIChE, Singapore.
• Examination of clavulanic acid biosynthesis using a comparative genomics approach 2018. AbuSara N., M. Moor M., Piercey B., Srivastava S.K, Ferguson N, Morales P, McIsaac C, Gomez F and Tahlan K. (Natural Product Discovery and Development in the Genomic Era conference, Clearwater Beach Florida USA)
• Srivastava, S. K, King, K., AbuSara, N., Malayny, C., Wilson, J. and Tahlan, K., 2018. Genetic and in vivo functional analysis of Ble (Orf12) from the clavulanic acid biosynthetic pathway of Streptomyces clavuligerus. Natural Product Discovery and Development in the Genomic Era, conference, Clearwater Beach Florida USA)
• Srivastava, S.K., Iyer, V.R., Ghosh, T., Lambadi, P.R., Pathania, R. and Navani, N.K., 2010. Selection of Combinatorial Promoter using in-vivo Selection Reveals Novel Sequence Which Respond to DNA Damaging Agents. (5th Science and Technology Congress at Dehradun (India).
• Srivastava, S.K., Iyer, V.R., Ghosh, T., Pathania, R. and Navani, N.K., 2010. Detection of Genotoxic Chemical Hazard Using Biosensors. International Conference on Challenges in Disaster mitigation and management (CDMM) IIT Roorkee (India)
• Bhattacharya T., Srivastava, S.K., Navani, N.K., Pathania, R. 2012. Small molecule screening identifies a novel cell division inhibitor with broad spectrum antibacterial potency. International Conference on Antimicrobial Research (ICAR), Lisbon (Portugal).

• Qualified All India Combined Entrance Examination (AICEE) for Biotechnology conducted by JNU Delhi held in May 2004.
• Recipient of scholarship for entire period of Master’s Course sponsored by Department of Biotechnology (DBT) Government of India.
• Qualified Graduate Aptitude Test in Engineering (GATE) –Biology stream (All India 141 rank (98.6 percentile)) conducted by Group of Indian Institute of Technologies (IITs) India.
• Recipient of Ministry of Human Resource and Development (MHRD), Government of India fellowship during PhD tenure.