Ruba Cynthia Rosaline Sebastian
Summary
Experienced microbiologist Specializing in microbial analysis and water quality management. Skilled in water testing, pathogen identification, and ensuring compliance with regulatory standards. Proficient in traditional microbiological techniques, including culture based methods and biochemical assays. Strong expertise in quality control,data interpretation and reporting to ensure water safety & quality. Committed to sustainable water resources management and public health protection.
Overview
24
24
years of professional experience
Work History
Private Tutor for Students
Private Home
01.2021 - Current
- Collaborated with students to complete homework assignments, identify lagging skills, and correct weaknesses.
- Met with parents to discuss student progress and address concerns.
- Monitored student performance through regular assessments, identifying strengths and areas for improvement.
- Supported students with helpful study habits and exam strategies.
- Used positive reinforcement, repetition, and review to help students master challenging material.
Trainee and Relief Assistant Teacher
Iyad Perdaus Childcare
01.2021 - 12.2021
- Assisted in maintaining a clean and orderly classroom environment, ensuring compliance with health and safety regulations while supporting an optimal learning space for students.
- Sanitized toys and play equipment each day to maintain safety and cleanliness.
- Participated in on-the-job training, working closely with supervisors and coworkers and asking appropriate questions.
- Attended training courses to build understanding of processes, techniques, and industry best practices in early childhood education, incorporating new strategies into the classroom as appropriate.
- Managed approximately 30 students in the classroom to verify safe and secure environment.
- Developed and implemented behavior management plans.
- Assisted in preparation of meals and snacks to provide children with proper nutrition.
- Helped new preschool children get used to classes by giving one-on-one support.
- Encouraged use of technology to support student learning.
Microbiologist, Laboratory Quality Control
J.N. Aqua Farms
04.2010 - 12.2012
- Conducted microbial analysis of water samples, including E. coli, Coliforms, Pseudomonas aeruginosa, sulfur-reducing anaerobes, and aerobic microbial counts.
- Performed yeast and mold counts to monitor water safety and quality.
- Analyzed physical and chemical parameters, including pH, Total Dissolved Solids (TDS), alkalinity, Chlorine (RFC), turbidity and taste.
- Maintained accurate records and prepared detailed reports for stakeholders on water quality findings.
- Operated and calibrated basic laboratory equipment for microbiological and chemical analyses.
- Contributed to maintaining water safety and public health through efficient quality monitoring programs.
Assistant Teacher
Christ the King Nursery and Primary School
06.2001 - 06.2002
- Responded to student needs and questions promptly.
- Promoted a positive learning environment by effectively managing classroom behavior and addressing individual needs.
- Assisted teachers in preparing instructional materials, ensuring alignment with curriculum objectives.
- Supported teacher in monitoring students during lunch periods, recess, and other non-instructional times to maintain safety and orderliness.
- Supported student physical, mental, and social development using classroom games and activities.
- Assisted in organizing and supervising school events and field trips, ensuring safe and educational experience for all students.
- Boosted classroom management skills, maintaining order and discipline among students.
- Fostered positive and supportive classroom atmosphere, encouraging student participation and confidence.
Education
Associate of Science - Microbiology
Singapore Polytechnic
Singapore04.2025
Skills
- Familiarity with water treatment methods( filtration, reverse osmosis, ion exchange)
- Analyze and report water quality like iron, pH, conductivity and other components
- Data analyze and reporting
- Microbial culture techniques(Isolation,Identification)
- Sterilization and disinfection procedures
- Microscopy and Staining techniques(Gram staining and Acid fast staining)
- Handling and maintenance of laboratory equipment
- Aseptic techniques and contamination control
- Basic molecular biology techniques
- Antibiotic Susceptibility Testing
- Cell Culturing
- Time Management
- Good laboratory practices
- Laboratory Management
- Microbial identification
- Quality control procedures
- Proficient in PCR
- DNA and RNA sequencing
- Pathogen detection
- DNA and RNA isolation
- RNA extraction and DNA extraction
- Gel electrophoresis
- Primer design
- Laboratory safety
- Time management abilities
- Sterilization techniques
- Bio safety protocols and procedures
Additional Information
Equipment Handled:
- Autoclave, Incubators,Microscope,Laminar Air Flow, Hot air Oven, Water Bath, Centrifuge
- Colony Counter, pH meter, Turbidity Meter, Membrane Filtration Unit, Petri Dishes and Test tube,
- Micro pipettes, Electronic Pipettes, Glass pipettes Spectrophotometer & Potable water analyzer
Timeline
Private Tutor for Students
Private Home
01.2021 - Current
Trainee and Relief Assistant Teacher
Iyad Perdaus Childcare
01.2021 - 12.2021
Microbiologist, Laboratory Quality Control
J.N. Aqua Farms
04.2010 - 12.2012
Assistant Teacher
Christ the King Nursery and Primary School
06.2001 - 06.2002
Associate of Science - Microbiology
Singapore Polytechnic
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