MUFEEDA MADATHUMMAL
Singapore
Summary
Dedicated Scientist with solid foundation in scientific research and development. Sharp and focused professional offering skills in electron Microscopy and Protein Biochemistry. Successfully led multiple projects resulting in significant advancement in protein biochemistry with a collaborative mindset.
Overview
14
14
years of professional experience
1
1
Certification
Work History
Scientist
A*STAR
07.2023 - 09.2024
- Successfully executed Cryo-Transmission Electron Microscope (Cryo-TEM), RT-TEM and Scanning Electron Microscope (SEM) service and collaboration projects to understand the drug targets, to study the effect of molecules on cell ultra-structure and to understand the effect of potential drug molecules, on bacteria, yeast and other organisms.
- Characterized extracellular vesicles and liposomes using TEM to investigate and understand underlying biological phenomena.
- Performed complex troubleshooting and assisted in leading initiatives to solve challenging scientific problems to improve the quality of protein for structural studies.
- Prepared reports summarizing research results for presentation to colleagues and management.
- Maintained accurate records of experimental protocols and results in accordance with regulatory requirements.
- Conducted literature reviews to support research projects and identify new areas of exploration.
Adjunct Research Fellow
National University of Singapore, NUS
04.2023 - 09.2024
- Proven expertise in protein expression, production, and characterization, with a focus on challenging soluble and membrane proteins that are difficult to express and stabilize.
- Proven ability to solve problems, with a solid understanding of construct design, biochemistry, and protein structure.
- Experience in the purification of proteins for target validation, screening and structure determination.
- Collaborated with multidisciplinary teams to tackle complex research questions from various perspectives.
Senior Research Fellow
A*SATR
04.2023 - 06.2023
- Accomplished implementation of TEM and SEM service initiatives and collaborative projects
- Played a key role in establishing the Cryo-EM lab, enhancing facility capabilities
- Led training and supervised a diverse team in electron microscopy, ensuring proficiency and effective lab operations
Research Fellow
A*SATR
10.2019 - 03.2023
- Proven track record of successfully executing range of TEM and SEM service and collaboration projects.
- Developed a protocol to differentiate between full and empty AAV vectors for TEM.
- Characterized quality of AAV gene vectors for gene therapy by TEM.
- Extensive experience using SEM to analyze the morphology of artificial meat and biomaterial, such as hydrogels, combined with a strong understanding of imaging techniques and material characterization.
- Maintained accurate records of experimental procedures, observations, and findings for transparent reporting of results in publications.
Doctoral Research
Nanyang Technological University
08.2014 - 01.2019
- Investigated the structural organization of Myosin Regulatory Light Chain (RLC) in the muscle cross-bridge by Transmission Electron Microscope
- Validated exogenously expressed protein exchanged muscle fibers using physiological and biochemical experiments.
- Performed cloning of the relevant genes and purified the proteins using AKTA chromatography.
- Conducted enzyme kinetic studies to assess the activity of the conjugated protein, contributing to a deeper understanding of its functional dynamics.
- Evaluated novel technologies for potential integration into ongoing studies.
- Mentored junior researchers, providing guidance on study design, and interpretation of results.
Junior Research Fellow
Indian Institute of Technology (IIT)
12.2010 - 04.2012
- Conducted genetic dissection of pattern formation in the cellular slime mold Polysphondylium pallidum.
- Identified the gene responsible for pattern formation through mutation studies, inverse PCR, and Southern blotting.
- Expert in using fluorescent microscope to identify the gene expression.
Education
Ph.D. - Biochemistry and Structural Biology
Nanyang Technological University
Singapore01.2019
Skills
- Transmission Electron Microscopy
- Scanning Electron Microscopy
- ELISA
- Cell Culture
- Protein Expression
- Purification from mammalian cells
- Purification from recombinant bacteria
- Chromatography (FPLC)
- Enzyme Kinetics
- SDS-PAGE
- Western blot
- EM software- EPU
- EM software- IMOD
- Biostatistics- Prism
- Biostatistics- R
- MS Excel
- Research Management
- Experiment Protocol Development
- Safety Processes and Procedures
- Project Management
- Routine Laboratory Operations
- Recordkeeping
Accomplishments
- GATE 2014, All India Rank 107, 99.1 percentile
- CSIR NET Lectureship Award, 06/01/12
- DBT Junior Research Fellowship, New Delhi, 01/01/09
- UGC Fellowship, Life Science, 01/01/09
Certification
Project Management Fundamentals
A*STAR Leadership & Organization Development (L&OD) Division
Issued Jun 2023
Publications
- Comparative analysis of sensory, textural, microstructural, amino acids and protein digestibility properties of animal and alternative meat products in the Asian market, International meat product in the Asian market, 07/01/24
- IPS-cell-derived microglia promote brain organoid maturation via cholesterol transfer, Nature, 11/01/23
- Functionalizing Insoluble Pea Protein Aggregates Using High-Pressure Homogenization: Effects on Physicochemical, Microstructural, and Functional Properties, Food Structure, 10/01/22
- Functional and Molecular Characterization of Heart Failure Progression in Mice and the Role of Myosin Regulatory Light Chains in the Recovery of Cardiac Muscle Function, International Journal of Molecular Sciences, 12/01/21
Languages
Malayalam
English
Tamil
References
References available upon request.
Work Availability
monday
tuesday
wednesday
thursday
friday
saturday
sunday
morning
afternoon
evening
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Work Preference
Work Type
Full TimeWork Location
On-SiteImportant To Me
Career advancementPersonal development programsWork-life balanceCompany CultureTimeline
Scientist
A*STAR
07.2023 - 09.2024
Adjunct Research Fellow
National University of Singapore, NUS
04.2023 - 09.2024
Senior Research Fellow
A*SATR
04.2023 - 06.2023
Research Fellow
A*SATR
10.2019 - 03.2023
Doctoral Research
Nanyang Technological University
08.2014 - 01.2019
Junior Research Fellow
Indian Institute of Technology (IIT)
12.2010 - 04.2012
Project Management Fundamentals
A*STAR Leadership & Organization Development (L&OD) Division
Issued Jun 2023
Ph.D. - Biochemistry and Structural Biology
Nanyang Technological University
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