Michelle Andrea Lian

Obesity Therapy through Lactate Clearance

• Investigated obesity therapy through lactate clearance, analyzing metabolic pathways and therapeutic interventions.
• Performed cell culture, component extraction, and Western blot to assess protein expression and metabolic markers.
• Characterized cell properties using SEM and DLS, evaluating structural and functional changes.
• Conducted biochemical assays for organ function and metabolite quantification to assess lactate clearance efficiency.

Application of Novel Natural Deep Eutectic Solvents for Protein Extraction

• Developed novel natural deep eutectic solvents (NADES) by optimizing component selection, molar ratios, and synthesis conditions, confirmed through Fourier Transform Infrared Spectroscopy (FTIR).
• Applied NADES for protein extraction from microalgae using a heat-assisted stirring method, followed by centrifugation and UV-Vis spectrophotometry to quantify extraction efficiency.
• Gained hands-on experience in analytical techniques and experimental design, including solvent synthesis, spectroscopy (FTIR, UV-Vis), protein quantification (Bradford assay), and data interpretation for process optimization.