Haripriya Sadhasivan

- Undertook the required coursework and curricular requirements towards the graduate degree

- Wrote novel protocols and optimized them for regular in vitro cell culture and maintenance

- Developed and standardized screening assays to test novel compounds that have potential therapeutic properties against hepatitis B virus

- Working on collaborative projects to establish the optimal conditions for in vitro infection models of hepatitis B

- Co-ordinate and liaise with relevant personnel with regard to lab maintenance, procurement and safety matters

• Involved in plasmid cloning, preparation of competent bacterial and mammalian cells, quality check and downstream applications of various plasmids
• Utilized cell culture techniques to help the post-doctoral researchers in carrying out their experiments on cell infection
• Immunization of BALB/c mice with viral proteins and evaluating the antibody production by conducting western blots
• Periodic collection of serum from mice for immunofluorescence assay
• Regular processing of clinical samples (swabs and blood) for the study and storing them for further use
• Isolation of PBMCs and separation of plasma and serum from human blood samples
• Managing the regular lab supplies and communicating with vendors to procure consumables, maintaining documentations for safety audits for regular functioning of the lab

• Involved in plasmid vector construction, preparation of competent bacterial cells, bacterial culturing, plasmid and minicircle isolation from the parent cells, quality check and downstream applications of the plasmids
• Utilized cell culture techniques to carry out various assays including FACS, q-RT-PCR, Western Blot, ELISA assays and tissue staining to determine the therapeutic effect of the extracellular vesicles on our target diseases
• Extensively worked on C57BL6 mice to establish an orthotopic bladder cancer model and a subcutaneous tumor model and to treat the tumour using EVs loaded with plasmids
• Prepared and presented the work in progress to supervising professor and colleagues on a bimonthly basis
• Managing the regular supplies and organizing the daily functioning of the lab

• Helped with preliminary data collection, sample processing and microbiological examination of the stool samples collected for surveillance
• Helped with preparing a systematic review on prevalence of antimicrobial resistant gut pathogens for the timeline of the past two decades
• Worked on data analytic tools such as R, STATA and Python for meta-analysis