Summary
Overview
Work History
Education
Skills
Websites
Hobbies
Awards
Publications
Languages
Timeline
Generic
Aruna Kalyanasundaram

Aruna Kalyanasundaram

Summary

Dedicated Protein Biochemist with specialization in Protein Characterization techniques. I am inclined towards AI application in protein biology.

Overview

14
14
years of professional experience

Work History

Life Science Cosnultant

Independant
08.2023 - Current


  • Spearheading consulting in cell culture and scale up (induced pluripotent stem cells (iPSC), mammalian) alongside artificial tissue and organ development.
  • Formulating comprehensive strategies encompassing the design of experiments (DoE), project management methodologies, budget allocation frameworks, and developing protocols.
  • Orchestrating successful partner meetings, leveraging adept negotiation skills to forge strategic alliances and propel project advancement.

Scientist

Medical Center
11.2020 - 03.2023
  • The project is at the Research Division of Cardiology, Gottingen medical center. Optimized the process of affinity purification- Mass spectrometer analysis reducing the time to 10x times thereby saving 50% cost.
  • The position focuses on metabolic phenotype between wild type and genetic knockout models in mouse and human stem cells
  • Cell culture of human stem cells (hiPSC) and cell-based assay development for metabolic studies
  • Protein- protein interaction study in mouse ventricular cardiomyocytes & human induced pluripotent stem cells (hiPSC)-ventricular cardiomyocytes by Co-immunoprecipitation and Mass Spectrometer based proteomics
  • Protein colocalization by Immunofluorescence, Confocal Microscopy.

Senior Scientist

Yacht.
09.2017 - 03.2019
  • The position focus on protein-glycan and protein-peptide drug product development along with characterization by analytical methods
  • Independently working on developing protein-peptide vaccine conjugates
  • Characterizing the protein using techniques such as SDS PAGE, Capillary gel electrophoresis (CGE ,Bioanalyzer 2100), pI estimation by cIEF, Western Blot
  • Particle size, Rheology and aggregation determination by Light scattering techniques such as Multi angled light scattering (SEC-MALS), Single light scattering (SLS), Dynamic light scattering (DLS), Melting temperature determination using Differential scanning fluorometry (nanoDSF)
  • Used Design of Experiments (DOE) for process optimization
  • Protein purification by AKTA Avant (Anion exchange chromatography), Size exclusion chromatography
  • Can independently use Unicorn, chromeleon, Empower tools
  • Preparation of SOP, Working Instructions for CRO and Technology transfer.

Analytical Scientist

Takeda Vaccines.
02.2012 - 01.2013
  • The company was focused on discovering and early development of Denvax, a viral vaccine for Dengue fever
  • My job profile included Vero cell culture for obtaining extracellular protein, which was used as a part of the vaccine for the Dengue serotypes 1,2,3,4
  • Analytical testing of routine samples of dengue IgM using ELISA
  • Method development, qualification and validation of protocol by, virus neutralization assays, HPLC (Shimadzu), Western Blot, SDS PAGE, cell based assays
  • Tech transfer: Writing Tech Transfer and Validation/Verification Documents
  • Used design of experiment (DoE), statistical tools for analytical analysis
  • Performed equipment maintenance and validation report, prepared IQ/OQ/PQ documents
  • Writing technical reports, making presentations, record keeping, and lab maintenance.

Upstream Scientist Grade II

Therapeutics Proteins Inc
10.2009 - 10.2010
  • Therapeutics protein, a company working on producing biosimilars dedicated on treating life threatening diseases such as cancer
  • My major focus was on mammalian cell culture, scale up and bacterial cell culture
  • Microbial (E.Coli) fermentation and Mammalian (CHO cells) cell culture up to 50L
  • Process measurement and optimisation involves monitoring of pH, dissolved oxygen, cell density and metabolic analyses
  • Development and testing of optimal media composition for mammalian cell culture and scale up using Wave Bioreactor- 10/20, 20/50EH, preparing FDA compliance cell bank (clean room class 100,000)
  • Transfection of CHO DG44 cells
  • Routine testing for bacterial, mycoplasma contamination
  • Prepared SOPs, batch records for cell culture process and equipment in compliance with GMP practices
  • Performed plasmid extraction, purification, transformation of bacteria
  • Written, executed and assisted validation or calibration projects related to the upstream operation and equipment maintenance
  • Prepared IQ/OQ/PQ documents
  • Supervised clean room maintenance, Class 10000, 100000- Cleaning, particle counting, and documentation
  • I can independently handle several analytical techniques such HPLC (Agilent 1100/1200), UV- Visible spectrophotometer, Iso electric focusing, ELISA, SDS PAGE (reducing, non-reducing condition) and western blot.

Education

Master of Science, Biological Science -

Illinois Institute of Technology
United States Of America
07.2009

Bachelor of Engineering, Industrial Biotechnology -

Anna University
India
08.2006

Skills

  • Cell culture, Protein Purification & Characterization, Immunology
  • Statistical tools for Design of experiments (DOE)-PRISM, Artificial Intelligence
  • Project Management Software- PRINCE 2
  • Microsoft Office - MS Word, MS Excel, Power point
  • Lab management, Lean Six Sigma

Hobbies

  • Swimming
  • Cycling

Awards

  • Dean's scholarship for excellence- Illinois Institute of Technology, Chicago, IL, USA., 2009
  • Best Outstanding Student of Department of Biotechnology - Anna University, India., 2006

Publications

PMID: 19191267, DOI: 10.1002/sca.20133

PMID: 19640539, DOI: 10.1016/j.jbiomech.2009.05.031

PMID: 24187495, DOI: 10.2147/IJN.S52132 

PMID: 32545195, DOI: 10.3390/ijms21114166

Languages

English
Bilingual or Proficient (C2)
German
Beginner (A1)

Timeline

Life Science Cosnultant

Independant
08.2023 - Current

Scientist

Medical Center
11.2020 - 03.2023

Senior Scientist

Yacht.
09.2017 - 03.2019

Analytical Scientist

Takeda Vaccines.
02.2012 - 01.2013

Upstream Scientist Grade II

Therapeutics Proteins Inc
10.2009 - 10.2010

Master of Science, Biological Science -

Illinois Institute of Technology

Bachelor of Engineering, Industrial Biotechnology -

Anna University

Similar Profiles

CREATE PROFILE
Aruna Kalyanasundaram